PB10073

antibody from Boster Biological Technology

Targeting: KDM5B CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

PB10073

Provider

Boster Biological Technology

Product name

Anti-KDM5B/PLU1/Jarid1B Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for JARID1B/KDM5B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, IHC-P, ICC/IF, FCM. Reactive species: Human;Mouse;Rat;Monkey. JARID1B/KDM5B information: Molecular Weight: 175658 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level).

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of KDM5B using anti-KDM5B antibody (PB10073). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KDM5B antigen affinity purified polyclonal antibody (Catalog # PB10073) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KDM5B at approximately 180KD. The expected band size for KDM5B is at 180KD.

Additional image