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LearnMA5-24626
antibody from Invitrogen Antibodies
Targeting: KDM5B
CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Other assay [1]
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- Product number
- MA5-24626 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDM5B Monoclonal Antibody (CL1129)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DGINSLERKL KRRLEREGLS SERWERVKKM RTPKKKKIKL SHPKDMNNFK LERERSYELV RSAETHSLPS DTSYSEQEDS E Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 91%. Binds to an epitope located within the peptide sequence DGINSLERKLKRRLE as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL1129
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references KDM5B overexpression predicts a poor prognosis in patients with squamous cell carcinoma of the head and neck.
Huang D, Qiu Y, Li G, Liu C, She L, Zhang D, Chen X, Zhu G, Zhang X, Tian Y, Liu Y
Journal of Cancer 2018;9(1):198-204
Journal of Cancer 2018;9(1):198-204
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM5B in Lane1: Marker (kDa), Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: KDM5B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401985). Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of KDM5B in MCF7 cells using KDM5B Monoclonal Antibody (CL1129) (Product # MA5-24626), showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human breast cancer tissue shows strong nuclear immunoreactivity in tumor cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human colorectal cancer shows moderate immunoreactivity in cancer cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human prostate cancer tissue shows strong nuclear positivity in tumor cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human prostate shows moderate immunoreactivity in glandular cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human uterus tissue shows moderate nuclear positivity in glandular cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human liver tissue shows absence of immunoreactivity in hepatocytes (negative control). Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KDM5B in human stomach cancer tissue shows moderate to strong nuclear positivity in tumor cells. Samples were probed using a KDM5B Monoclonal Antibody (Product # MA5-24626).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
