A301-813A

antibody from Invitrogen Antibodies

Targeting: KDM5B CT31, JARID1B, PLU-1, PPP1R98, RBBP2H1A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-813A

Provider

Invitrogen Antibodies

Product name

JARID1B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human JARID1B by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1B antibody (Product # A301-813A; lot A301-813A-3) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Jarid1B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1B antibody A301-813A (lot A301-813A-2) used for IP at 6 µg per reaction. Jarid1B was also immunoprecipitated by a previous lot of this antibody (lot A301-813A-1) and rabbit anti-Jarid1B antibody BL4377 For blotting immunoprecipitated Jarid1B, A301-813A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Jarid1B by western blot. Samples: Whole cell lysate (50, 15, 5 µg) from 293T and (50 µg) HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1B antibody A301-813A (lot A301-813A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human JARID1B by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Rabbit anti-JARID1B antibody (Product # A301-813A; lot 3) used at 1:500 (2 µg/mL). Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Jarid1B by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Jarid1B (Cat. No. A301-813A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human JARID1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1B antibody (Product # A301-813A; lot A301-813A-3) used for IP at 6 µg per reaction. JARID1B was also immunoprecipitated by a previous lot of this antibody (lot A301-813A-2). For blotting immunoprecipitated Jarid1B (Product # A301-813A) was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.