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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC6686G - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Myogenin Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Myogenin in direct ELISAs and mouse Myogenin in flow cytometry.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
P15173- Isotype
- IgG
- Antibody clone number
- 671038
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Mesenchymal Stem Cells From Infants Born to Obese Mothers Exhibit Greater Potential for Adipogenesis: The Healthy Start BabyBUMP Project.
Platelet-rich plasma, especially when combined with a TGF-β inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
Boyle KE, Patinkin ZW, Shapiro AL, Baker PR 2nd, Dabelea D, Friedman JE
Diabetes 2016 Mar;65(3):647-59
Diabetes 2016 Mar;65(3):647-59
Platelet-rich plasma, especially when combined with a TGF-β inhibitor promotes proliferation, viability and myogenic differentiation of myoblasts in vitro.
Kelc R, Trapecar M, Gradisnik L, Rupnik MS, Vogrin M
PloS one 2015;10(2):e0117302
PloS one 2015;10(2):e0117302
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Myogenin in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse Myogenin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC6686G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
