PAB3188

antibody from Abnova Corporation

Targeting: MAP4K1 HPK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3188

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3188, RRID:AB_1577189

Product name

MAP4K1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of MAP4K1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Hematopoietic progenitor kinase 1 (HPK1) negatively regulates prostaglandin E2-induced fos gene transcription.

Sawasdikosol S, Russo KM, Burakoff SJ
Blood 2003 May 1;101(9):3687-9

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Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.

Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH
Genes & development 1996 Sep 15;10(18):2251-64

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MAP4K1 polyclonal antibody (Cat # PAB3188) in A-375 cell lysate. MAP4K1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAP4K1 polyclonal antibody (Cat # PAB3188) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)