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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB8125 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8125, RRID:AB_1710088
- Product name
- MAP4K1 (phospho S171) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP4K1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Molecular & cellular proteomics : MCP 2007 Mar;6(3):537-47
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH
Genes & development 1996 Sep 15;10(18):2251-64
Genes & development 1996 Sep 15;10(18):2251-64
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of MAP4K1 (phospho S171) polyclonal antibody (Cat # PAB8125) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
