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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB100-1045 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1045, RRID:AB_10002900
- Product name
- Goat Polyclonal MAP4K1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH
Genes & development 1996 Sep 15;10(18):2251-64
Genes & development 1996 Sep 15;10(18):2251-64
Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade.
Hu MC, Qiu WR, Wang X, Meyer CF, Tan TH
Genes & development 1996 Sep 15;10(18):2251-64
Genes & development 1996 Sep 15;10(18):2251-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MAP4K1 Antibody [NB100-1045] - Staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MAP4K1 Antibody [NB100-1045] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
