HPA007419

antibody from Atlas Antibodies

Targeting: MAP4K1 HPK1

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA007419

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA007419, RRID:AB_1853778

Product name

Anti-MAP4K1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

LDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTH
RSSSLGIPDADCCRRHMEFRKLRGMETRPPANTAR
LQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAED
TPPPLPPKPKFRSPS


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human lymph node and pancreas tissues using HPA007419 antibody. Corresponding MAP4K1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows positivity in germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows positivity in germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows positivity in lymphoid cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas shows no positivity as expected.

Sample type

HUMAN