H00004194-B01P

antibody from Abnova Corporation

Targeting: MDM4 HDMX, MDMX

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004194-B01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004194-B01P, RRID:AB_10731201

Product name

MDM4 purified MaxPab mouse polyclonal antibody (B01P)

Antibody type

Polyclonal

Description

Mouse polyclonal antibody raised against a full-length human MDM4 protein.

Antigen sequence

MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLK
ILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQE
QHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKN
LVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSA
EESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLI
ENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNY
TPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESV
SEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGK
NDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSP
SKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIP
EKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKL
FDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQ
RTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTG
HLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Stress-induced isoforms of MDM2 and MDM4 correlate with high-grade disease and an altered splicing network in pediatric rhabdomyosarcoma.

Jacob AG, O'Brien D, Singh RK, Comiskey DF Jr, Littleton RM, Mohammad F, Gladman JT, Widmann MC, Jeyaraj SC, Bolinger C, Anderson JR, Barkauskas DA, Boris-Lawrie K, Chandler DS
Neoplasia (New York, N.Y.) 2013 Sep;15(9):1049-63

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MDM4 expression in transfected 293T cell line (H00004194-T01) by MDM4 MaxPab polyclonal antibody.Lane 1: MDM4 transfected lysate(53.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to MDM4 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol