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Learn22026-1-AP
antibody from Invitrogen Antibodies
Targeting: NPHP3
CFAP31, FLJ30691, FLJ36696, KIAA2000, MKS7, NPH3, SLSN3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- 22026-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NPHP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGTASSLVS PAGGEVIEDT YGAGGGEACE IPVEVKPKAR LLRNSFRRGA GAAAGAGPGS LPRGVGAGGL LGASFKSTGS SVPELEYAAA EYERLRKEYE IFRVSKNQEL LSMGRREAKL DTENKRLRAE LQ (1-131 aa encoded by BC068082)
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 22026-1-AP (NPHP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result (the base and the lower half of cilia stain) of anti-NPHP3 (22026-1-AP, 1:50) with serum-starved hTERT-RPE1 (MeOH fixed) by Dr. Moshe Kim.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 22026-1-AP ( NPHP3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 22026-1-AP ( NPHP3 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-NPHP3 (IP:22026-1-AP, 4ug; Detection:22026-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
