H00079858-M01

antibody from Abnova Corporation

Targeting: NEK11 FLJ23495

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00079858-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00079858-M01, RRID:AB_426078

Product name

NEK11 monoclonal antibody (M01), clone 4E1-1F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant NEK11.

Antigen sequence

MLKFQEAAKCVSGSTAISTYPKTLIARRYVLQQKL
GSGSFGTVYLVSDKKAKRGEELKVLKEISVGELNP
NETVQANLEAQLLSKLDHPAIVKFHASFVEQDNFC
IITEYCEGRDLDDKIQEYKQAGKIFPENQIIEWFI
QLLLGVDYMHERRILHRDLKSKNVFLKNNLLKIGD
FGVSRLLMGSCDLATTLTGTPHYMSPEALKHQGYD
TKSDIWSLACILYEMCCMNHAFAGSNFLSIVLKIV
EGDTPSLPERYPKELNAIMESMLNKNPSLRPSAIE
ILKIPYLDEQLQNLMCRYSEMTLEDKNLDCQKEAA
HIINAMQKRIHLQTLRALSEVQKMTPRERMRLRKL
QAADEKARKLKKIVEEKYEENSKRMQELRSRNFQQ
LSVDVLHEKTHLKGMEEKEEQPEGRLSCSPQDEDE
ERWQGREEESDEPTLENLPESQPIPSMDLHELESI
VEDATSDLGYHGDCNLISLDEYWKNEK


Isotype

IgG

Antibody clone number

4E1-1F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NEK11 monoclonal antibody (M01), clone 4E1-1F4 Western Blot analysis of NEK11 expression in HL-60 ( Cat # L014V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

NEK11 monoclonal antibody (M01), clone 4E1-1F4. Western Blot analysis of NEK11 expression in A-431 ( Cat # L015V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody (M01), clone 4E1-1F4.Lane 1: NEK11 transfected lysate(55.5 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged NEK11 is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol