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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501608 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501608, RRID:AB_11124416
- Product name
- Anti-NEK11 mouse monoclonal antibody, clone OTI5E9 (formerly 5E9)
- Antibody type
- Monoclonal
- Description
- Anti-NEK11 mouse monoclonal antibody, clone OTI5E9 (formerly 5E9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- NEK11
- Isotype
- IgG
- Antibody clone number
- OTI5E9
- Vial size
- 100 µl
- Concentration
- 0.76 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK11 (RC221953, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK11.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC221953 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NEK11 antibody(TA501608), and then analyzed by flow cytometry.
- Validation comment
- FC
