PA5-17680

antibody from Invitrogen Antibodies

Targeting: RPS6KA3 CLS, HU-3, MRX19, RSK2

Antibody data

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Validation data

RPS6KA3 protein structure - PA5-17680 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Ribosomal protein S6 kinase alpha-1 was achieved by transfecting HEK-293 with Ribosomal protein S6 kinase alpha-1 specific siRNAs (Silencer® select Product # S12274, S12273). Western Blot analysis (Fig. a) was performed using Whole cell extracts from the Ribosomal protein S6 kinase alpha-1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The Blot was probed with RSK1/RSK2/RSK3 Polyclonal Antibody (Product # PA5-17680, 1:1000) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western Blot is shown in histogram (Fig. b).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-RSK1/RSK2/RSK3 Polyclonal Antibody (Product # PA5-17680) and a 80 kDa band corresponding to Ribosomal protein S6 kinase alpha-1 was observed. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), K-562 (Lane 3), A-431 (Lane 4), HEL 92.1.7 (Lane 5) and BJ (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).