Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-17900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RSK1 (Ser380) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Canine, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 30 µg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot was performed using Anti-Phospho-RSK1 (Ser380) Polyclonal Antibody (Product # PA5-17900) and a 80 kDa band corresponding to Phosphorylated Ribosomal protein S6 kinase alpha-1 was observed. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with PMA (Over-night serum starvation followed by treatment with 25 ng/mL PMA for 20 mins) (Lane 2) and HeLa treated with EGF (Over-night serum starvation followed by treatment with 100 nM EGF for 10 mins) (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Up-regulation in the expression of phosphorylated RSK1 was observed in the lanes treated with PMA and EGF as expected (https://doi.org/10.1038/onc.2012.472, doi: 10.1091/mbc.E11-07-0658).