Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF2056 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal RSK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human, mouse and rat RSK family isoforms in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Integrins promote cytokinesis through the RSK signaling axis.
Mathew SS, Nieves B, Sequeira S, Sambandamoorthy S, Pumiglia K, Larsen M, Laflamme SE
Journal of cell science 2014 Feb 1;127(Pt 3):534-45
Journal of cell science 2014 Feb 1;127(Pt 3):534-45
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse RSK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.