GTX10696

antibody from GeneTex

Targeting: RPS6KA1 HU-1, MAPKAPK1, p90Rsk, RSK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX10696

Provider

GeneTex

Proper citation

GeneTex Cat#GTX10696, RRID:AB_381139

Product name

p90 RSK1 (phospho Ser363) antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide (Human).

Host

Rabbit

Isotype

IgG

Vial size

50µl

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from A431 cells left unstimulated (1) or stimulated with 100 ng/mL PMA (a phorbol ester activator of protein kinase C) for 30 minutes at 37oC (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 3% Milk-TBST buffer for one hour at room temperature and incubated with RSK1 [pS363]/2 [pS369] antibody for one hour at room temperature in a 1% Milk-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate in 1% Milk-TBST buffer, and bands were detected. The data show induction of the RSK1 [pS363]/2 [pS369] antibody signal by PMA treatment and that the peptide corresponding to this site blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Validation comment

WB