AP09419PU-S

antibody from OriGene

Targeting: RPS6KA1 HU-1, MAPKAPK1, p90Rsk, RSK1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

AP09419PU-S

Provider

OriGene

Product name

RSK1 p90 (RPS6KA1) pSer732 rabbit polyclonal antibody, Aff - Purified

Antibody type

Polyclonal

Description

RSK1 p90 (RPS6KA1) pSer732 rabbit polyclonal antibody, Aff - Purified

Host

Rabbit

Conjugate

Unconjugated

Epitope

RPS6KA1

Isotype

IgG

Antibody clone number

NULL

Vial size

25 µl

Concentration

0.64 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using affinity purified antip90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.

Validation comment

WB