PAB8134

antibody from Abnova Corporation

Targeting: RPS6KA1 HU-1, MAPKAPK1, p90Rsk, RSK1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB8134

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB8134, RRID:AB_1709942

Product name

RPS6KA1 (phospho S363) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RPS6KA1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Tumor-promoting phorbol esters and activated Ras inactivate the tuberous sclerosis tumor suppressor complex via p90 ribosomal S6 kinase.

Roux PP, Ballif BA, Anjum R, Gygi SP, Blenis J
Proceedings of the National Academy of Sciences of the United States of America 2004 Sep 14;101(37):13489-94

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p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1.

Bohuslav J, Chen LF, Kwon H, Mu Y, Greene WC
The Journal of biological chemistry 2004 Jun 18;279(25):26115-25

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90-kDa ribosomal S6 kinase is a direct target for the nuclear fibroblast growth factor receptor 1 (FGFR1): role in FGFR1 signaling.

Hu Y, Fang X, Dunham SM, Prada C, Stachowiak EK, Stachowiak MK
The Journal of biological chemistry 2004 Jul 9;279(28):29325-35

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of RPS6KA1 (phospho S363) polyclonal antibody (Cat # PAB8134) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)