PAB16907

antibody from Abnova Corporation

Targeting: RPS6KA1 HU-1, MAPKAPK1, p90Rsk, RSK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB16907

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB16907, RRID:AB_10677891

Product name

RPS6KA1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RPS6KA1 .

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Subcellular localization and biological actions of activated RSK1 are determined by its interactions with subunits of cyclic AMP-dependent protein kinase.

Chaturvedi D, Poppleton HM, Stringfield T, Barbier A, Patel TB
Molecular and cellular biology 2006 Jun;26(12):4586-600

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using RPS6KA1 polyclonal antibody (Cat # PAB16907) shows detection of a band ~90 KDa in size corresponding to RPS6KA1 (arrowhead) in EGF stimulated (Lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (Lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1 : 1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.