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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MA5-24701 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RSK1 (Thr359, Ser363) Recombinant Rabbit Monoclonal Antibody (RM233)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts to human RSK1 only when phosphorylated at Thr359/Ser363. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- RM233
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of A431 cells treated (+) or nontreated (-) with EGF, usingPhospho-RSK1 (Thr359, Ser363) Antibody (Product # MA5-24701) at a 1:1000 dilution, showed a band of phosphorylated Rsk1 only in EGF treated A431 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of A431 cells treated (+) or nontreated (-) with EGF, usingPhospho-RSK1 (Thr359, Ser363) Antibody (Product # MA5-24701) at a 1:1000 dilution, showed a band of phosphorylated Rsk1 only in EGF treated A431 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed and paraffin embedded Human cerebral cortex tissue sections using Phospho-RSK1 (Thr359, Ser363) Antibody (Product # MA5-24701) at a 1:200 dilution.
