Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C745338 - Provider product page
- Provider
- LSBio
- Product name
- RPS6KA1 / RSK1 Antibody (phospho-Ser732) LS-C745338
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Horse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-p90 RSK1 pS732 antibody. Lane 1-2: HEK293T (U) untreated or (T) treated. Lane 3-4: HEK293T (U) untreated or (T) treated. Lane 5-6: HEK293T (U) untreated or (T) treated. Load: 15µg per lane. Actin used as a loading control. Blocking: 5% milk. Primary Antibody: Anti-RSK1-pS732 1:1000, 1:2000, or 1:3000 O/N. Secondary Antibody: Goat Anti-Rabbit IgG 1:5000 for 2 hours. Predicted Size: ~90 kDa in size corresponding to phosphorylated p90 RSK1 in EGF stimulated.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL.