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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-17841 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RSK1 (Thr573) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 38 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-p90RSK (pThr573) was performed by loading 20 µg of HEK293T whole cell lysates, from cells either left untreated (Lane 1) or induced with 100 nM PMA (Lane 2) for 15 minutes, per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat dry milk in TBST for 1 hour at room temperature. The membrane was probed with a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) at a dilution of 1:250 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:40,000 for 1 hour at room temperature. Detection was performed using ECL substrate. Data courtesy of the Innovators Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-p90RSK pThr573 in HeLa cells, TPA-treated, using a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) (green). Actin filaments are labeled with a fluorescent red phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-p90RSK pThr573 in HeLa cells using a Phospho-p90RSK pThr573 polyclonal antibody (Product # PA5-17841) (green). Actin filaments are labeled with a fluorescent red phalloidin.
