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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-21699 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RSK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2. Predicted reactivity: Mouse (97%), Rat (95%), Pig (98%), Chicken (91%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.47 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RSK1 Polyclonal Antibody detects p90 RSK1 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract.7.5% SDS-PAGE. RSK1 Polyclonal Antibody (Product # PA5-21699) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RSK1 Polyclonal Antibody detects p90 RSK1 protein at Cytoplasm and Nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p90 RSK1 protein stained by RSK1 antibody (Product # PA5-21699) diluted at 1:500. Blue: Hoechst 33343 staining.
