PA5-29215

HU-1, MAPKAPK1, p90Rsk, RSK1

Antibody data

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Validation data

Product number: PA5-29215
Provider: Invitrogen Antibodies
Product name: Anti-RSK1 Polyclonal Antibody
Provider product page: Invitrogen Antibodies - PA5-29215
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: PA5-29215 targets RSK1 in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-29215 immunogen is recombinant protein fragment corresponding to a region within amino acids 47 and 276 of Human RSK1. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of RSK1 using 30 µg of HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RSK1 polyclonal antibody (Product # PA5-29215) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of RSK1 was achieved by transfecting HeLa with RSK1 specific siRNAs (Silencer® select Product # s12273; s12274). Western blot analysis (Fig. a) was performed using whole cell extracts from the RSK1 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RSK1 Polyclonal Antibody (Product # PA5-29215, 1:5000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RSK1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extract (1% SDS) (30 µg lysate) of A-431 (Lane 1), HeLa (Lane 2), THP-1 (Lane 3), Hep G2 (Lane 4), MOLT-4 (Lane 5), SK-OV-3 (Lane 6) and Mouse Ovary (Lane 7). The blot was probed with Anti-RSK1 Polyclonal Antibody (Product # PA5-29215, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 81 kDa band corresponding to RSK1 was detected in all cell lines tested with a non-specific band (*) at ~40 kDa.