Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB120-5777 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-5777, RRID:AB_2253960
- Product name
- Rabbit Polyclonal RSK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: RSK1 [p Ser380] Antibody [NB120-5777] - Lysates prepared from A431 cells left unstimulated (1) or stimulated with PMA for 30min at 37C (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with 5% BSA-TBST buffer for 1hr at RT and incubated with RSK1 [pS380]/2 [pS386] antibody for 1hr at RT in 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), non-phosphopeptide corresponding to the immunogen (3), generic phosphoserine-containing peptide (4) or the phosphopeptide immunogen (5). Data show induction of the RSK1 [pS380]/2 [pS386] Ab signal by PMA treatment and that the peptide corresponding to this site blocks the Ab signal, thereby demonstrating the specificity of the Ab.