Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-12-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TGF-beta 2 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects TGF-beta 2 and TGF-beta 1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with TGF-beta 3 and TGF-beta 1 is observed.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references MicroRNA-30b controls endothelial cell capillary morphogenesis through regulation of transforming growth factor beta 2.
Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells.
Cancer-associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-β-dependent pathways in genotype-specific oral cancer.
Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
Isoform specificity of commercially-available anti-TGF-beta antibodies.
Isoform specificity of commercially-available anti-TGF-beta antibodies.
Howe GA, Kazda K, Addison CL
PloS one 2017;12(10):e0185619
PloS one 2017;12(10):e0185619
Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells.
Reithmeier A, Panizza E, Krumpel M, Orre LM, Branca RMM, Lehtiö J, Ek-Rylander B, Andersson G
BMC cancer 2017 Sep 15;17(1):650
BMC cancer 2017 Sep 15;17(1):650
Cancer-associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-β-dependent pathways in genotype-specific oral cancer.
Cirillo N, Hassona Y, Celentano A, Lim KP, Manchella S, Parkinson EK, Prime SS
Carcinogenesis 2017 Jan;38(1):76-85
Carcinogenesis 2017 Jan;38(1):76-85
Differential versican isoforms and aggrecan expression in the chicken embryo aorta.
Arciniegas E, Neves CY, Candelle D, Parada D
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 2004 Jul;279(1):592-600
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 2004 Jul;279(1):592-600
Isoform specificity of commercially-available anti-TGF-beta antibodies.
Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93
Journal of immunological methods 1999 May 27;225(1-2):87-93
Isoform specificity of commercially-available anti-TGF-beta antibodies.
Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93
Journal of immunological methods 1999 May 27;225(1-2):87-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TGF-beta 2 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 2 at approximately 64 kDa (as indicated) using 5 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TGF-beta 2 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TGF-beta 2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- TGF-beta 2 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 2 Antibody. Porcine TGF-beta 2 (Catalog # 102-B2) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 2 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-TGF-beta 2 Polyclonal Antibody (Catalog # AB-12-NA). The ND50 is typically 0.25-1.25 µg/mL.