PA5-77966
antibody from Invitrogen Antibodies
Targeting: EPRS1
EARS, EPRS, GLUPRORS, PARS, QARS, QPRS
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-77966 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EPRS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Chicken (84%), Bovine (90%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Drosophila
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.23 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EPRS in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with EPRS polyclonal antibody (Product # PA5-77966) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EPRS Polyclonal Antibody detects EPRS protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with EPRS Polyclonal Antibody (Product # PA5-77966) diluted at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of EPRS was performed in 293T whole cell extracts using 5 µg of EPRS Polyclonal Antibody (Product # PA5-77966). Samples were transferred to a membrane and probed with EPRS Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.