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- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- 11472-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#11472-1-AP, RRID:AB_10755306
- Product name
- Calpain 2 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Calpain 2 antibody (Cat. #11472-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells.
Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.
Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.
Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.
Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction.
Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp.
Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.
TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
Mechanosensitive Piezo1 channels mediate renal fibrosis.
Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca(2+) Signaling Pathway.
Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.
The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.
Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.
Xu M, Zhong W, Yang C, Liu M, Yuan X, Lu T, Li D, Zhang G, Liu H, Zeng Y, Yang X, Zhou Y, Zhou L
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 May;127:155392
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 May;127:155392
Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.
Wang WY, Shi JX, Chen MH, Zhuge XZ, Dai CG, Xie L
Experimental and therapeutic medicine 2023 May;25(5):196
Experimental and therapeutic medicine 2023 May;25(5):196
Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.
Liu X, Jiang L, Zhang W, Zhang J, Luan X, Zhan Y, Wang T, Da J, Liu L, Zhang S, Guo Y, Zhang K, Wang Z, Miao N, Xie X, Liu P, Li Y, Jin H, Zhang B
Journal of translational medicine 2023 Jun 27;21(1):417
Journal of translational medicine 2023 Jun 27;21(1):417
Aberrant mechanical loading induces annulus fibrosus cells apoptosis in intervertebral disc degeneration via mechanosensitive ion channel Piezo1.
Liu C, Gao X, Lou J, Li H, Chen Y, Chen M, Zhang Y, Hu Z, Chang X, Luo M, Zhai Y, Li C
Arthritis research & therapy 2023 Jul 7;25(1):117
Arthritis research & therapy 2023 Jul 7;25(1):117
Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction.
Marshall KD, Klutho PJ, Song L, Roy R, Krenz M, Baines CP
Journal of the American Heart Association 2023 Feb 21;12(4):e025867
Journal of the American Heart Association 2023 Feb 21;12(4):e025867
Zinc deficiency causes oxidative stress, endoplasmic reticulum stress, apoptosis and inflammation in hepatocytes in grass carp.
Cui J, Xu T, Lv H, Guo MY
Fish & shellfish immunology 2023 Aug;139:108905
Fish & shellfish immunology 2023 Aug;139:108905
Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.
Chen CL, Wang HL, Qian F, Liu MH, Guo XX, Lv J, Huang JH, Zhang NZ, Xiang Z
Frontiers in pharmacology 2022;13:939039
Frontiers in pharmacology 2022;13:939039
TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.
Tian W, Liu SY, Zhang M, Meng JR, Tang N, Feng YD, Sun Y, Gao YY, Zhou L, Cao W, Li XQ
Pharmacological research 2022 Jul;181:106262
Pharmacological research 2022 Jul;181:106262
Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.
Wang J, Gao F, Cui S, Yang S, Gao F, Wang X, Zhu G
Pharmacological research 2022 Feb;176:106079
Pharmacological research 2022 Feb;176:106079
Mechanosensitive Piezo1 channels mediate renal fibrosis.
Zhao X, Kong Y, Liang B, Xu J, Lin Y, Zhou N, Li J, Jiang B, Cheng J, Li C, Wang W
JCI insight 2022 Apr 8;7(7)
JCI insight 2022 Apr 8;7(7)
Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca(2+) Signaling Pathway.
Liu L, Zhang Q, Xiao S, Sun Z, Ding S, Chen Y, Wang L, Yin X, Liao F, Jiang LH, Xue M, You Y
Frontiers in pharmacology 2021;12:606245
Frontiers in pharmacology 2021;12:606245
Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.
Song Z, Bian Z, Zhang Z, Wang X, Zhu A, Zhu G
Toxicology and applied pharmacology 2021 Oct 15;429:115711
Toxicology and applied pharmacology 2021 Oct 15;429:115711
The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.
Kotani T, Takegaki J, Tamura Y, Kouzaki K, Nakazato K, Ishii N
Physiological reports 2021 May;9(9):e14842
Physiological reports 2021 May;9(9):e14842
Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.
Wang WY, Xie L, Zou XS, Li N, Yang YG, Wu ZJ, Tian XY, Zhao GY, Chen MH
International immunopharmacology 2021 Apr;93:107377
International immunopharmacology 2021 Apr;93:107377
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.
Wang S, Huang Y, Yan Y, Zhou H, Wang M, Liao L, Wang Z, Chen D, Ji D, Xia X, Liu F, Huang J, Xiong K
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2019 Jan;221:57-67
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 11472-1-AP(CAPN2 antibody) at dilution of 1:200
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 11472-1-AP(CAPN2 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
