TA504345

antibody from OriGene

Targeting: CAPN2 CANPL2, CANPml, mCANP

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA504345

Provider

OriGene

Proper citation

OriGene Cat#TA504345, RRID:AB_11124339

Product name

CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Antibody type

Monoclonal

Description

CAPN2 (Calpain 2) mouse monoclonal antibody, clone OTI1F4 (formerly 1F4)

Host

Mouse

Conjugate

Unconjugated

Epitope

CAPN2

Isotype

IgG

Antibody clone number

OTI1F4

Vial size

100 µl

Concentration

0.75 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN2 (RC205642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN2.

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC205642 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-CAPN2 antibody(TA504345), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-CAPN2 antibody(TA504345),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC