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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Product number
- M03492 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Calpain 2 CAPN2 Antibody Picoband™ (monoclonal, 8I6)
- Antibody type
- Monoclonal
- Description
- Mouse IgG monoclonal antibody for Calpain 2 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Monkey.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8I6
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of Calpain 2 using anti- Calpain 2 antibody (M03492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: U20S whole cell lysates, Lane 3: COS-7 whole cell lysates, Lane 4: K562 whole cell lysates, Lane 5: HELA whole cell lysates, Lane 6: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Calpain 2 antigen affinity purified monoclonal antibody (Catalog # M03492) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Calpain 2 at approximately 80KD. The expected band size for Calpain 2 is at 80KD.
- Additional image
