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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Flow cytometry [3]
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- Product number
- NBP2-01814 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Calpain 2 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.75 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpain 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpain 2.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calpain 2 Antibody (1F4) [NBP2-01814] Analysis of extracts (35ug) from 9 different cell lines by using anti-Calpain 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Hela cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - Analysis of Jurkat cells, using anti-Calpain 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Calpain 2 Antibody (1F4) [NBP2-01814] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Calpain 2 antibody, and then analyzed by flow cytometry.
