- PA5-29381 - Invitrogen Antibodies CAPN2 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Calpain 2 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Calpain 2 polyclonal antibody (Product # PA5-29381) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Calpain 2 Polyclonal Antibody (Product # PA5-29381). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Calpain 2 Polyclonal Antibody (Product # PA5-29381). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of Calpain 2 (CAPN2) was achieved by transfecting A-431 with Calpain 2 (CAPN2) specific siRNAs (Silencer® select Products # s321). Western blot analysis (Fig. a) was performed using whole cell extracts from the Calpain 2 (CAPN2) knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Calpain 2 Polyclonal Antibody (Product # PA5-29381, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CAPN2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-Calpain 2 Polyclonal Antibody (Product # PA5-29381) and a 76 kDa band corresponding to Calpain 2 was observed in DU 145, A-431 and PC-3, but not in C2C12, NIH/3T3 and Daudi. Whole cell lysates (30 µg lysate) of C2C12 (Lane 1), DU 145 (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-3 (Lane 5), and Daudi (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Calpain 2 Polyclonal Antibody detects Calpain 2 protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Calpain 2 (Product # PA5-29381) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-29381