PA5-29381

antibody from Invitrogen Antibodies

Targeting: CAPN2 CANPL2, CANPml, mCANP

Antibody data

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Validation data

Product number: PA5-29381 - Provider product page
Provider: Invitrogen Antibodies
Product name: Calpain 2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, mouse brain. Predicted reactivity: Mouse (91%), Rat (91%), Pig (94%), Rabbit (93%), Chicken (86%), Sheep (94%), Rhesus Monkey (98%), Bovine (93%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.47 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CAPN2 protein structure - PA5-29381 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Calpain 2 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Calpain 2 polyclonal antibody (Product # PA5-29381) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Calpain 2 Polyclonal Antibody (Product # PA5-29381). Sample (50 µg of whole cell lysate). Lane A: Mouse brain . 7.5% SDS PAGE. Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Calpain 2 Polyclonal Antibody (Product # PA5-29381). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 2 Polyclonal Antibody (Product # PA5-29381) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Calpain 2 (CAPN2) was achieved by transfecting A-431 with Calpain 2 (CAPN2) specific siRNAs (Silencer® select Products # s321). Western blot analysis (Fig. a) was performed using whole cell extracts from the Calpain 2 (CAPN2) knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Calpain 2 Polyclonal Antibody (Product # PA5-29381, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CAPN2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Calpain 2 Polyclonal Antibody (Product # PA5-29381) and a 76 kDa band corresponding to Calpain 2 was observed in DU 145, A-431 and PC-3, but not in C2C12, NIH/3T3 and Daudi. Whole cell lysates (30 µg lysate) of C2C12 (Lane 1), DU 145 (Lane 2), NIH/3T3 (Lane 3), A-431 (Lane 4), PC-3 (Lane 5), and Daudi (Lane 6) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).