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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- Ab086727 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant 53BP2/ASPP2 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human 53BP2/ASPP2 Antibody, Recombinant (R09-1B5), could be used for WB and so on.ApplicationWB: 1/500-1/1000Protein Function Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 20-84- Antibody clone number
- R09-1B5
- Vial size
- 100μl,10μl,1ml,50μl
- Concentration
- 0,33 mg/ml
- Storage
- Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant 53BP2/ASPP2 Antibody (Ab086727) - Western Blot All lanes: Recombinant 53BP2/ASPP2 Antibody (Ab086727) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 126 kDa Observed band size: 110, 146 kDa Exposure time: 2.6 s
