PA1-12912

antibody from Invitrogen Antibodies

Targeting: DEGS1 DEGS-1, Des-1, DES1, FADS7, MLD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12912

Provider

Invitrogen Antibodies

Product name

DEGS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control for this product is placenta lysate.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Degerative Spermatocyte Homolog 1 (DSH1) was performed by loading 40 µg of mouse retinal tissue lysates from 5 individual mice: 3 wild type mice (WT), where the photoreceptors are predominantly rods; and 2 mutant mice (Mutant), where the photoreceptors are predominantly cones per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in TBST for 1h RT. The membrane was probed with a DSH1 polyclonal antibody (Product # PA1-12912) at a dilution of 1:1000 4°C, washed, and probed with an HRP-conjugated anti-rabbit IgG secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature. Detection was performed using ECL substrate (Product # 32106). Data courtesy of the Innovators Program.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on 4% paraformaldehyde-fixed frozen mouse retina tissue sections. Tissues were blocked in 3% normal goat serum + 0.3% Triton X-100 for 1 hour at room temperature and probed with a Degenerative Spermatocyte Homolog 1 (DSH1) polyclonal antibody (Product # PA1-12912) at a dilution of 1:500 overnight at 4°C. Tissues were washed extensively with PBS. Detection was performed using a Cy3-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:500. Nuclei (blue) were stained with DAPI, and staining was visualized by fluorescence microscopy. Data courtesy of the Innovators Program.