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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB2032 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2032, RRID:AB_1677670
- Product name
- LEFTY2 monoclonal antibody, clone 7C5G1H6H10
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody raised against recombinant LEFTY2.
- Isotype
- IgG
- Antibody clone number
- 7C5G1H6H10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Lefty inhibits receptor-regulated Smad phosphorylation induced by the activated transforming growth factor-beta receptor.
Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1.
lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal.
Ulloa L, Tabibzadeh S
The Journal of biological chemistry 2001 Jun 15;276(24):21397-404
The Journal of biological chemistry 2001 Jun 15;276(24):21397-404
Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1.
Tsukui T, Capdevila J, Tamura K, Ruiz-Lozano P, Rodriguez-Esteban C, Yonei-Tamura S, Magallón J, Chandraratna RA, Chien K, Blumberg B, Evans RM, Belmonte JC
Proceedings of the National Academy of Sciences of the United States of America 1999 Sep 28;96(20):11376-81
Proceedings of the National Academy of Sciences of the United States of America 1999 Sep 28;96(20):11376-81
lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal.
Meno C, Shimono A, Saijoh Y, Yashiro K, Mochida K, Ohishi S, Noji S, Kondoh H, Hamada H
Cell 1998 Aug 7;94(3):287-97
Cell 1998 Aug 7;94(3):287-97
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- LEFTY2 monoclonal antibody, clone 7C5G1H6H10 (Cat # MAB2032) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY2.Detection occurs after 1.0 ug of protein is loaded in each lane.The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody, clone 7C5G1H6H10 at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1 : 1,000.Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag.Lane 2 contains partially purified recombinant human LEFTY.The antibody may be used to detect endogenous human LEFTY.
