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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R1173 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1173, RRID:AB_1004554
- Product name
- anti LEFTY2
- Antibody type
- Monoclonal
- Antigen
- A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7C5G1H6H10
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant6X His tagged human LEFTY. Detection occurs after 1.0 µg of protein is loaded in each lane. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at room temperaturefor 30 min followed by detection using IRDye(TM) 800 labeled Goata-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 containspartially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark ofLI-COR, Inc. Other detection systems will yield similar results.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- R1173 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Uterus, Myometrium at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.
