21645-1-AP

antibody from Proteintech Group

Targeting: ROCK2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21645-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21645-1-AP, RRID:AB_10858624

Product name

ROCK2(middle) antibody

Antibody type

Polyclonal

Description

KD/KO validated ROCK2(middle) antibody (Cat. #21645-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Tongfu-Xingshen Capsule ameliorates ischemic stroke by inhibiting pyroptosis-mediated neuroinflammation.

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Journal of ethnopharmacology 2026 Jul 15;366:121565

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Excessive hypercholesterolemia in pregnancy impairs the cardiovascular health of the adult female and male offspring.

Graton ME, de Oliveira AA, Neupane A, Kirschenman R, Quon A, Spaans F, Cooke CM, Davidge ST
Bioscience reports 2026 Jan 9;46(1)

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Rho-related GTP-binding protein RhoE (RND3) regulates multiple myeloma bone disease.

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Simulated microgravity affects neuronal synaptic plasticity by regulating microglial pro-inflammatory activation.

Chen X, Yuan C, Li Z, Zhao T, Yang Y, Wang Y, Qiao C, Liu Z, Li X
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TAGLN-RhoA/ROCK2-SLC2A3-mediated Mechano-metabolic Axis Promotes Skin Fibrosis.

Cheng X, Gao Z, Zhang J, Zheng H, Shan S, Zhou J
International journal of biological sciences 2025;21(2):658-670

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Chinese massage therapy (Tuina) inhibits motor neuron apoptosis in rats with sciatic nerve injury by regulating the cPLA2 and RhoA/ROCK2 signaling pathways.

Sun J, Zhang Y, Liu Z, Zhang H, Liu J, Xu Y, Na R, Zhang H, Yan J, Yu T
Frontiers in neurology 2025;16:1622602

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Effect of four traditional Chinese medicine monomers on mechanical barrier damage and inflammation response of IPEC-J2 cells caused by soybean 7S globulin.

Deng Y, Wu X, Wan Y, Deng J, Deng H
Frontiers in veterinary science 2025;12:1548866

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Neutrophil-derived heparin-binding protein increases endothelial permeability in acute lung injury by promoting TRIM21 and the ubiquitination of P65.

Zhang J, Cao Y, Shu W, Dong S, Sun Y, Ma X
Cell biology and toxicology 2025 Mar 5;41(1):55

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In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment.

He W, Ding F, Zhang L, Liu W
Nature communications 2025 Jan 30;16(1):1162

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Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer.

Du X, Luo W, Li H, Gu Q, Huang P, Wang C, Li N, Liu F, Xia C
Molecular cancer 2025 Feb 27;24(1):59

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Sevoflurane-induced overexpression of extrasynaptic α5-GABA(A)R via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Wang Z, Dong J, Zhang M, Wang S, Wu J, Wang S, Luo Y, Wang Y, Yin Y
Aging cell 2024 Sep;23(9):e14209

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Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway.

Fang H, Fan LL, Ding YL, Wu D, Zheng JY, Cai YF, Huang Y, Qiao LJ, Zhang SJ, Zhan J
Neurochemical research 2024 Nov;49(11):3105-3117

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Fasudil Alleviates Postoperative Neurocognitive Disorders in Mice by Downregulating the Surface Expression of α5GABAAR in Hippocampus.

Dong J, Wang Z, Li L, Zhang M, Wang S, Luo Y, Dong Y, Wang X, Wang Y, Wang K, Yin Y
CNS neuroscience & therapeutics 2024 Nov;30(11):e70098

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PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2-dependent control of the actin cytoskeleton, modulating emotional behavior.

Yu L, Zeng F, Fan M, Zhang K, Duan J, Tan Y, Liao P, Wen J, Wang C, Wang M, Yuan J, Pang X, Huang Y, Zhang Y, Li JD, Zhang Z, Hu Z
Zoological research 2024 May 18;45(3):535-550

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Therapeutic potential of Lingjiao Gouteng decoction in acute alcohol intoxication and alcohol-induced brain injury involving the RhoA/ROCK2/NF-κB signaling pathway.

Li J, Liu Y, Chen X, Luo M, Yin M, Xie X, Ai Y, Zhang X, He J
Journal of ethnopharmacology 2024 Jun 28;328:118114

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Fasudil attenuates oxidative stress-induced partial epithelial-mesenchymal transition of tubular epithelial cells in hyperuricemic nephropathy via activating Nrf2.

Cao Y, Wang Y, Li W, Feng J, Chen Y, Chen R, Hu L, Wei J
European journal of pharmacology 2024 Jul 15;975:176640

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ROCK2-RNA interaction map reveals multiple biological mechanisms underlying tumor progression in renal cell carcinoma.

Hong Z, Chen X, Wang L, Zhou X, He H, Zou G, Liu Q, Wang Y
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MicroRNA-30a-3p: a potential noncoding RNA target for the treatment of arteriosclerosis obliterans.

Zhang M, Chen Y, Niu F, Luo X, Li J, Hu W
Aging 2023 Oct 27;15(21):11875-11890

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Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.

Yang J, Wang L, Zhang Z, Sun Q, Zhang Y
International journal of molecular medicine 2023 Jul;52(1)

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Nrf2 attenuates methamphetamine-induced myocardial injury by regulating oxidative stress and apoptosis in mice.

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Xia J, Li J, Deng M, Yin F, Liu J, Wang J
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The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

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Oxycodone relieves permeability damage and apoptosis of oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cells through ras homolog family member A (RhoA)/ Rho-associated coiled-coil containing kinases (ROCK)/ myosin light chain 2 (MLC2) signal.

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Acquired temozolomide resistance in MGMT(low) gliomas is associated with regulation of homologous recombination repair by ROCK2.

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Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis.

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Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.

Wang Y, Jiang L, Mo X, Lan Y, Yang X, Liu X, Zhang J, Zhu L, Liu J, Wu X
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FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

Cui K, Ruan Y, Wang T, Rao K, Chen Z, Wang S, Liu J
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Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents.

Zhu J, Xu M, Gao M, Zhang Z, Xu Y, Xia T, Liu S
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Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Ching RH, Lau EY, Ling PM, Lee JM, Ma MK, Cheng BY, Lo RC, Ng IO, Lee TK
Oncotarget 2015 Dec 22;6(41):43483-95

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Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.

Huang D, Du X, Yuan R, Chen L, Liu T, Wen C, Huang M, Li M, Hao L, Shao J
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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP(ROCK2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ROCK2 (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue