Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00009475-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009475-M02, RRID:AB_922377
- Product name
- Mouse Monoclonal ROCK2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ROCK2 (1E12)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Aortic smooth muscle cell alterations in mice systemically exposed to arsenic.
Vascular hyperpermeability in response to inflammatory mustard oil is mediated by Rho kinase in mice systemically exposed to arsenic.
Chen SC, Huang SY, Lin WT, Yang RC, Yu HS
Heart and vessels 2016 May;31(5):807-15
Heart and vessels 2016 May;31(5):807-15
Vascular hyperpermeability in response to inflammatory mustard oil is mediated by Rho kinase in mice systemically exposed to arsenic.
Chen SC, Liu CC, Huang SY, Chiou SJ
Microvascular research 2011 Sep;82(2):182-9
Microvascular research 2011 Sep;82(2):182-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ROCK2 Antibody (1E12) [H00009475-M02] - ROCK2 monoclonal antibody (M02), clone 1E12 Analysis of ROCK2 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ROCK2 Antibody (1E12) [H00009475-M02] - Detection limit for recombinant GST tagged ROCK2 is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on HeLa cell. Antibody concentration 30 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ROCK2 Antibody (1E12) [H00009475-M02] - Analysis of monoclonal antibody to ROCK2 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 1.5 ug/ml.