Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB12328 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12328, RRID:AB_10680825
- Product name
- ROCK2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ROCK2.
- Isotype
- IgG
- Storage
- Store at -20°C on dry atmosphere.After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Targeted disruption of the mouse rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death.
Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Thumkeo D, Keel J, Ishizaki T, Hirose M, Nonomura K, Oshima H, Oshima M, Taketo MM, Narumiya S
Molecular and cellular biology 2003 Jul;23(14):5043-55
Molecular and cellular biology 2003 Jul;23(14):5043-55
Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24.
Takahashi N, Tuiki H, Saya H, Kaibuchi K
Genomics 1999 Jan 15;55(2):235-7
Genomics 1999 Jan 15;55(2):235-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ROCK2 expression from cell extracts with ROCK2 polyclonal antibody (Cat # PAB12328).Lane 1 : MCF-7 whole cell lysate.Lane 2 : HeLa whole cell lysate.Lane 3 : smmc whole cell lysate.Lane 4 : HT1080 whole cell lysate.Lane 3 : MM453 whole cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ROCK2 on formalin fixed, paraffin embedded human intestinal cancer with ROCK2 polyclonal antibody (Cat # PAB12328).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)