A01023-1

antibody from Boster Biological Technology

Targeting: ROCK2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01023-1

Provider

Boster Biological Technology

Product name

Anti-ROCK2 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for ROCK2 detection. Tested with WB, ICC/IF, FCM, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

ROCK2 mediates the proliferation of pulmonary arterial endothelial cells induced by hypoxia in the development of pulmonary arterial hypertension.

Qiao F, Zou Z, Liu C, Zhu X, Wang X, Yang C, Jiang T, Chen Y
Experimental and therapeutic medicine 2016 Jun;11(6):2567-2572

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The role of Rho/Rho-kinase pathway and the neuroprotective effects of fasudil in chronic cerebral ischemia.

Yan YY, Wang XM, Jiang Y, Chen H, He JT, Mang J, Shao YK, Xu ZX
Neural regeneration research 2015 Sep;10(9):1441-9

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ROCK pathway participates in the processes that 15-hydroxyeicosatetraenoic acid (15-HETE) mediated the pulmonary vascular remodeling induced by hypoxia in rat.

Ma J, Liang S, Wang Z, Zhang L, Jiang J, Zheng J, Yu L, Zheng X, Wang R, Zhu D
Journal of cellular physiology 2010 Jan;222(1):82-94

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of A549 cells using anti-ROCK2 antibody (A01023-1). Overlay histogram showing A549 cells stained with A01023-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ROCK2 Antibody (A01023-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image