Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-39743 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-TAK1 (Ser439) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references TXNIP Regulates Natural Killer Cell-Mediated Innate Immunity by Inhibiting IFN-γ Production during Bacterial Infection.
Kim DO, Byun JE, Kim WS, Kim MJ, Choi JH, Kim H, Choi E, Kim TD, Yoon SR, Noh JY, Park YJ, Lee J, Cho HJ, Lee HG, Min SH, Choi I, Jung H
International journal of molecular sciences 2020 Dec 14;21(24)
International journal of molecular sciences 2020 Dec 14;21(24)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-TAK1/MAP3K7 pSer439 in extracts from Jurkat cells using a Phospho-TAK1/MAP3K7 pSer439 polyclonal antibody (Product # PA5-39743).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-TAK1/MAP3K7 pSer439 in paraffin-embedded human brain tissue using a Phospho-TAK1/MAP3K7 pSer439 polyclonal antibody (Product # PA5-39743).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 The loss of Txnip induces the activation of TAK1 and IFN-gamma production in NK cells during Pam3CSK4 stimulation. ( A ) Representative images of FACS histograms. WT and KO NK cells were cultured at 5 x 10 5 cells per well in 48-well plate and treated with Pam (1 µg/mL) for 1 h. WT NK cells; black line, KO NK cells; red line ( n = 3). Repeated three times. ( B ) WT and KO NK cells were cultured at 1-2 x 10 6 cells per well in 24-well plate and stimulated with Pam3CSK4 (1 µg/mL) for indicated time. The levels of TLR1/2 signaling molecules were analyzed by Western blotting. Repeated three times. ( C ) The production of IFN-gamma was blocked by TLR1/2 signaling inhibitor, CU CPT22, in NK cells ( n = 3). Repeated three times. ( D ) Knockdown of Txnip mRNA level in human primary NK cells was confirmed by quantitative real-time PCR at 42 h after electroporation ( n = 3). Repeated three times. ( E ) Production of IFN-gamma in human NK cells transfected with 300 nmol/100 uL of control or Txnip siRNA. NK cells were cultured at 1 x 10 6 cells per well in 24-well plate and treated by Pam3CSK4 (1 µg/mL) for 18 h ( n = 3). Repeated three times. ( F ) Representative confocal images for the phosphorylation of TAK1. Knockdown of Txnip induced the phosphorylation of TAK1 in human NK cells. NK cells were seeded on fibronectin-coated glass coverslips and treated with Pam (1 µg/mL) for 30 min. Repeated three times. ( G ) Measurement of mean fluorescent intensity