H00006885-M02

antibody from Abnova Corporation

Targeting: MAP3K7 MEKK7, TAK1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006885-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006885-M02, RRID:AB_436974

Product name

MAP3K7 monoclonal antibody (M02), clone 2E10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant MAP3K7.

Antigen sequence

MSTASAASSSSSSSAGEMIEAPSQVLNFEEIDYKE
IEVEEVVGRGAFGVVCKAKWRAKDVAIKQIESESE
RKAFIVELRQLSRVNHPNIVKLYGACLNPVCLVME
YAEGGSLYNVLHGAEPLPYYTAAHAMSWCLQCSQG
VAYLHSMQPKALIHRDLKPPNLLLVAGGTVLKICD
FGTACDIQTHMTNNKGSAAWMAPEVFEGSNYSEKC
DVFSWGIILWEVITRRKPFDEIGGPAFRIMWAVHN
GTRPPLIKNLPKPIESLMTRCWSKDPSQRPSMEEI
VKIMTHLMRYFPGADEPLQYPCQYSDEGQSNSATS
TGSFMDIASTNTSNKSDTNMEQVPATNDTIKRLES
KLLKNQAKQQSESGRLSLGASRGSSVESLPPTSEG
KRMSADMSEIEARIAATTGNGQPRRRSIQDLTVTG
TEPGQVSSRSSSPSVRMITTSGPTSEKPTRSHPWT
PDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSK
ESMAVFEQHCKMAQEYMKVQTEIALLLQRKQELVA
ELDQDEKDQQNTSRLVQEHKKLLDENKSLSTYYQQ
CKKQLEVIRSQQQKRQGTS


Isotype

IgG

Antibody clone number

2E10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody (M02), clone 2E10.Lane 1: MAP3K7 transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MAP3K7 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol