PAB0516

antibody from Abnova Corporation

Targeting: MAP3K7 MEKK7, TAK1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0516

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0516, RRID:AB_982695

Product name

MAP3K7 (phospho S192) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP3K7.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Wnt activates the Tak1/Nemo-like kinase pathway.

Smit L, Baas A, Kuipers J, Korswagen H, van de Wetering M, Clevers H
The Journal of biological chemistry 2004 Apr 23;279(17):17232-40

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Tumor necrosis factor-alpha-induced IKK phosphorylation of NF-kappaB p65 on serine 536 is mediated through the TRAF2, TRAF5, and TAK1 signaling pathway.

Sakurai H, Suzuki S, Kawasaki N, Nakano H, Okazaki T, Chino A, Doi T, Saiki I
The Journal of biological chemistry 2003 Sep 19;278(38):36916-23

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A dominant negative TAK1 inhibits cellular fibrotic responses induced by TGF-beta.

Ono K, Ohtomo T, Ninomiya-Tsuji J, Tsuchiya M
Biochemical and biophysical research communications 2003 Jul 25;307(2):332-7

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of MAP3K7 (phospho S192) polyclonal antibody (Cat # PAB0516) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.

Validation comment

Dot Blot (Peptide)