Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00006885-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006885-M02, RRID:AB_540436
- Product name
- Mouse Monoclonal TAK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MAP3K7 - mitogen-activated protein kinase kinase kinase 7
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68.
Rui Y, Su J, Wang H, Chang J, Wang S, Zheng W, Cai Y, Wei W, Gordy JT, Markham R, Kong W, Zhang W, Yu XF
Journal of virology 2017 Jul 1;91(13)
Journal of virology 2017 Jul 1;91(13)
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 over-expressed 293 cell line, cotransfected with MAP3K7 Validated Chimera RNAi ( Cat # H00006885-R02V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP3K7 monoclonal antibody (M02) clone 2E10 (Cat # H00006885-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TAK1 Antibody (2E10) [H00006885-M02] - Analysis of MAP3K7 expression in transfected 293T cell line by MAP3K7 monoclonal antibody (M02), clone 2E10.Lane 1: MAP3K7 transfected lysate(63.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TAK1 Antibody (2E10.) [H00006885-M02] - TAK1 Antibody (2E10) [H00006885-M02] - Detection limit for recombinant GST tagged MAP3K7 is approximately 1ng/ml as a capture antibody.
