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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-17507 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TAK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 29 μg/mL
- Storage
- -20°C
Submitted references Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.
Vallecillo-Zúniga ML, Poulson PD, Luddington JS, Arnold CJ, Rathgeber M, Kartchner BC, Hayes S, Gill H, Valdoz JC, Spallino JL, Garfield S, Dodson EL, Arthur CM, Stowell SR, Van Ry PM
Cells 2021 Nov 17;10(11)
Cells 2021 Nov 17;10(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of TAK1 in HeLa cells using a TAK1 polyclonal antibody (Product # PA5-17507) (blue) compared to a nonspecific negative control antibody (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 In vitro treatment with rHsGal-1 modulates inflammatory response through the NF-kappaB pathway. ( A ) Quantification of expression levels of p65 (normalized to beta-tubulin) in 48 h A/J -/- NT or 0.11 muM rHsGal-1 treated myotubes. ( B ) Western blot images showing the p65 expression in NT or 0.11 muM rHsGal-1 48 h A/J -/- treated myotubes. ( C ) Representative images of WT and NT or 0.11 muM rHsGal-1 48 h A/J -/- treated myotubes cultured and immunostained with p65 (green), Phalloidin (red), and DAPI (blue). ( D - H ) Western blot quantification of 48 h NT or 0.11 muM rHsGal-1treated myotubes expressing levels of TAK1 ( D ), NIK ( E ), IKBalpha ( F ), p50 ( G ), and P-p65 ( H ). ( I ) Western blot images of 48 h NT or 0.11 muM rHsGal-1treated myotubes expressing NF-kappaB inflammatory subunits quantified in D-H. n = 3 in each group. A. * p < 0.05 and ** p < 0.01 NT vs. all forms of Gal-1. D-H. * p < 0.05, ** p < 0.01, *** p < 0.001 NT vs. rHsGal-1. Data are represented as SEM.
