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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB3914 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat ERK4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat ERK4.
- Reactivity
- Human, Mouse, Rat
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P31152- Isotype
- IgG
- Antibody clone number
- 355810
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse ERK4 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ERK4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- ERK4 in Raji Human Cell Line. ERK4 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
