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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- HPA025073 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA025073, RRID:AB_1847695
- Product name
- Anti-SPRTN
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPRTN, Gene description: SprT-like N-terminal domain, Alternative Gene Names: C1orf124, DKFZP547N043, DVC1, Spartan, Validated applications: ICC, Uniprot ID: Q9H040, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Replication-associated formation and repair of human topoisomerase IIIα cleavage complexes
A Novel Approach: Investigating the Intracellular Clearance Mechanism of Glyceraldehyde-Derived Advanced Glycation End-Products Using the Artificial Checkpoint Kinase 1 d270KD Mutant as a Substrate Model
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability
SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells
SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
DVC1 (C1orf124) is a DNA damage–targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks
Saha L, Saha S, Yang X, Huang S, Sun Y, Jo U, Pommier Y
Nature Communications 2023;14(1)
Nature Communications 2023;14(1)
A Novel Approach: Investigating the Intracellular Clearance Mechanism of Glyceraldehyde-Derived Advanced Glycation End-Products Using the Artificial Checkpoint Kinase 1 d270KD Mutant as a Substrate Model
Takeda K, Sakai-Sakasai A, Kajinami K, Takeuchi M
Cells 2023;12(24):2838
Cells 2023;12(24):2838
The protease SPRTN and SUMOylation coordinate DNA-protein crosslink repair to prevent genome instability
Ruggiano A, Vaz B, Kilgas S, Popović M, Rodriguez-Berriguete G, Singh A, Higgins G, Kiltie A, Ramadan K
Cell Reports 2021;37(10):110080
Cell Reports 2021;37(10):110080
SPRTN protease-cleaved MRE11 decreases DNA repair and radiosensitises cancer cells
Na J, Newman J, Then C, Syed J, Vendrell I, Torrecilla I, Ellermann S, Ramadan K, Fischer R, Kiltie A
Cell Death & Disease 2021;12(2)
Cell Death & Disease 2021;12(2)
SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
Lopez-Mosqueda J, Maddi K, Prgomet S, Kalayil S, Marinovic-Terzic I, Terzic J, Dikic I
eLife 2016;5
eLife 2016;5
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
DVC1 (C1orf124) is a DNA damage–targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks
Mosbech A, Gibbs-Seymour I, Kagias K, Thorslund T, Beli P, Povlsen L, Nielsen S, Smedegaard S, Sedgwick G, Lukas C, Hartmann-Petersen R, Lukas J, Choudhary C, Pocock R, Bekker-Jensen S, Mailand N
Nature Structural & Molecular Biology 2012;19(11):1084-1092
Nature Structural & Molecular Biology 2012;19(11):1084-1092
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Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
- Sample type
- Human
