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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX50009 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX50009, RRID:AB_11176870
- Product name
- PHD2 antibody [AT21E9]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
