Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-36695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-TrkB (Tyr516) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 92 and 145 kDa.
- Concentration
- 1 mg/mL
Submitted references SLITRK2 variants associated with neurodevelopmental disorders impair excitatory synaptic function and cognition in mice.
Dimeric mimetic of BDNF loop 4 promotes survival of serum-deprived cell through TrkB-dependent apoptosis suppression.
Impact of a Histone Deacetylase Inhibitor-Trichostatin A on Neurogenesis after Hypoxia-Ischemia in Immature Rats.
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Therapeutic Hypothermia Provides Variable Protection against Behavioral Deficits after Neonatal Hypoxia-Ischemia: A Potential Role for Brain-Derived Neurotrophic Factor.
El Chehadeh S, Han KA, Kim D, Jang G, Bakhtiari S, Lim D, Kim HY, Kim J, Kim H, Wynn J, Chung WK, Vitiello G, Cutcutache I, Page M, Gecz J, Harper K, Han AR, Kim HM, Wessels M, Bayat A, Jaén AF, Selicorni A, Maitz S, de Brouwer APM, Silfhout AV, Armstrong M, Symonds J, Küry S, Isidor B, Cogné B, Nizon M, Feger C, Muller J, Torti E, Grange DK, Willems M, Kruer MC, Ko J, Piton A, Um JW
Nature communications 2022 Jul 15;13(1):4112
Nature communications 2022 Jul 15;13(1):4112
Dimeric mimetic of BDNF loop 4 promotes survival of serum-deprived cell through TrkB-dependent apoptosis suppression.
Zainullina LF, Vakhitova YV, Lusta AY, Gudasheva TA, Seredenin SB
Scientific reports 2021 Apr 8;11(1):7781
Scientific reports 2021 Apr 8;11(1):7781
Impact of a Histone Deacetylase Inhibitor-Trichostatin A on Neurogenesis after Hypoxia-Ischemia in Immature Rats.
Zalewska T, Jaworska J, Sypecka J, Ziemka-Nalecz M
International journal of molecular sciences 2020 May 27;21(11)
International journal of molecular sciences 2020 May 27;21(11)
Effect of the HDAC Inhibitor, Sodium Butyrate, on Neurogenesis in a Rat Model of Neonatal Hypoxia-Ischemia: Potential Mechanism of Action.
Jaworska J, Zalewska T, Sypecka J, Ziemka-Nalecz M
Molecular neurobiology 2019 Sep;56(9):6341-6370
Molecular neurobiology 2019 Sep;56(9):6341-6370
Therapeutic Hypothermia Provides Variable Protection against Behavioral Deficits after Neonatal Hypoxia-Ischemia: A Potential Role for Brain-Derived Neurotrophic Factor.
Diaz J, Abiola S, Kim N, Avaritt O, Flock D, Yu J, Northington FJ, Chavez-Valdez R
Developmental neuroscience 2017;39(1-4):257-272
Developmental neuroscience 2017;39(1-4):257-272
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Phospho-TrkB (Tyr516) was performed using 70% confluent log phase A549 cells, untreated or treated with 25 ng/mL BDNF for 1 hr. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Phospho-TrkB (Tyr516) Rabbit Polyclonal Antibody (Product # PA5-36695) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly cytosolic localization. Panel e represents control A549 cells without treatment, whereas Panel f represents no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-TrkB pTyr516 in paraffin-embedded human colorectal carcinoma using Phospho-TrkB pTyr516 polyclonal antibody (Product # PA5-36695) at a dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 10 Effect of TSA on the expression of TrkB and phospho-TrkB in the brain hemispheres after HI. Representative immunoblots of total TrkB protein ( A ) and phosphorylated TrkB ( C ) level. The intensity of each band was quantified and normalized in relation to beta-actin. The bar graphs ( B and D ) represent statistical analysis of immunoreactive bands estimated in indicated experimental groups: sham-control (Sham), TSA-treated sham-control (Sham+TSA), hypoxia-ischemia (HI), TSA-treated hypoxia-ischemia (HI+TSA). Note that TSA application increases the level of TrkB phosphorylation at 7 days of recovery. The values are mean +- SD from 5 animals per group and time point assessed in 3 independent experiments. Two-way ANOVA tests indicate significant differences between investigated groups, *** p < 0.001 (effect of HI insult), +++ p < 0.001 (effect of TSA treatment).. Abbreviations: ipsi--ipsilateral, contra--contralateral.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 18 Sodium butyrate increases the level of TrkB and phospho-TrkB in the ipsilateral hemisphere 7 days after HI. Representative immunoblots of total TrkB ( a ) and phospho-TrkB ( c ) protein. The intensity of each band obtained by respective Western blotting was quantified and normalized in relation to beta-actin. Bar graphs ( b and d ) represent statistical analysis of densitometric data presented as percent of control value from indicated experimental groups. The values are means +- SD from five animals per group and time point assessed in three independent experiments. One-way ANOVA and Bonferroni test indicate significant differences between investigated groups, * p < 0.05, *** p < 0.001. Abbreviations: C --control, ipsi --ipsilateral, contra--contralateral