MAB1494

antibody from Novus Biologicals

Targeting: NTRK2 TRKB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

MAB1494

Provider

Novus Biologicals

Product name

Rat Monoclonal TrkB Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects mouse TrkB in direct ELISAs. Detects human, mouse, and rat TrkB in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TrkA, recombinant rat TrkA, or recombinant mouse TrkC is observed.

Reactivity

Human, Mouse, Rat

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse TrkB by Western Blot. Western blot shows lysate of mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TrkB Monoclonal Antibody (Catalog # MAB1494) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for TrkB at approximately 90-100 and 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat TrkB by Western Blot. Detection of Human, Mouse, and Rat TrkB by Western Blot.